Overview
Type: Synthetic peptide
Form: Lyophilized Powder
ENPP1 (extracellular) Blocking Peptide (#BLP-NT069) is the original antigen used for immunization during Anti-ENPP1 (extracellular) Antibody (#ANT-069) generation. The blocking peptide binds and ‘blocks’ Anti-ENPP1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Anti-ENPP1 (extracellular) Antibody (#ANT-069), (1:400).
3-4. Anti-ENPP1 (extracellular) Antibody, preincubated with ENPP1 Blocking Peptide (BLP-NT069).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 4), human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 5) and human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 3 and 6):1-3. Anti-ENPP1 (extracellular) Antibody (#ANT-069), (1:200).
4-6. Anti-ENPP1 (extracellular) Antibody, preincubated with ENPP1 Blocking Peptide (BLP-NT069).
Expression of ENPP1 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-ENPP1 (extracellular) Antibody (#ANT-069), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the dorsal horn region, showed ENPP1 immunoreactivity (green) neuronal profiles (vertical arrows) and glial profiles (horizontal arrows). B. Pre-incubation of the antibody with ENPP1 Blocking Peptide (BLP-NT069), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide CLYQDFRIPLSPVHK, corresponding to amino acid residues 711 - 725 of human ENPP1 (Accession P22413).
Accession (Uniprot) Number P22413
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
