Overview
Type: Synthetic peptide
Form: Lyophilized powder
ENT3/SLC29A3 Blocking Peptide (#BLP-NT053) is the original antigen used for immunization during Anti-ENT3 (SLC29A3) Antibody (#ANT-053) generation. The blocking peptide binds and ‘blocks’ Anti-ENT3/SLC29A3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat liver lysate (lanes 1 and 4), mouse liver lysate (lanes 2 and 5) and rat kidney membranes (lanes 3 and 6):1-3. Anti-ENT3 (SLC29A3) Antibody (#ANT-053), (1:200).
4-6. Anti-ENT3 (SLC29A3) Antibody, preincubated with ENT3/SLC29A3 Blocking Peptide (#BLP-NT053).
Properties
Sequence
- (C)DYPAPGLQRPEDR, corresponding to amino acid residues 37-49 of rat ENT3 (Accession Q80WK7).
Accession (Uniprot) Number Q80WK7
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
