Overview
Type: Synthetic peptide
Form: Lyophilized powder
EphA1 (extracellular) Blocking Peptide (#BLP-ER011) is the original antigen used for immunization during Anti-EphA1 (extracellular) Antibody (#AER-011) generation. The blocking peptide binds and ‘blocks’ Anti-EphA1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-EphA1 (extracellular) Antibody (#AER-011), (1:200).
3,4. Anti-EphA1 (extracellular) Antibody, preincubated with EphA1 (extracellular) Blocking Peptide (#BLP-ER011).
Western blot analysis of human Jurkat T cell leukemia cells (lanes 1 and 5), human HeLa cervix adenocarcinoma cells (lanes 2 and 6), human SH-SY5Y neuroblastoma cells (lanes 3 and 7) and human MCF-7 breast adenocarcinoma cells (lanes 4 and 8):1-4. Anti-EphA1 (extracellular) Antibody (#AER-011), (1:200).
5-8. Anti-EphA1 (extracellular) Antibody, preincubated with EphA1 (extracellular) Blocking Peptide (#BLP-ER011).
Expression of EphA1 in rat striatum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-EphA1 (extracellular) Antibody (#AER-011), (1:300), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. EphA1 immunoreactivity (red) appears in striatal neurons (arrows). B. Pre-incubation of the antibody with EphA1 (extracellular) Blocking Peptide (#BLP-ER011), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KKEPRQLELTWAGSR, corresponding to amino acid residues 457-471 of human EphA1 (Accession P21709).
Accession (Uniprot) Number P21709
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
