Overview
Type: Synthetic peptide
Form: Lyophilized Powder
EphA2 (extracellular) Blocking Peptide (#BLP-ER019) is the original antigen used for immunization during Anti-EphA2 (extracellular) Antibody (#AER-019) generation. The blocking peptide binds and ‘blocks’ Anti-EphA2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1, 2. Anti-EphA2 (extracellular) Antibody (#AER-019), (1:200).
3, 4. Anti-EphA2 (extracellular) Antibody, preincubated with EphA2 (extracellular) Blocking Peptide (#BLP-ER019).
Western blot analysis of human U87-MG glioblastoma cell line lysate (lanes 1 and 5), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 6), human LNCaP prostate adenocarcinoma cell line lysate (lanes 3 and 7) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 4 and 8):1-4. Anti-EphA2 (extracellular) Antibody (#AER-019), (1:200).
5-8. Anti-EphA2 (extracellular) Antibody, preincubated with EphA2 (extracellular) Blocking Peptide (#BLP-ER019).
Expression of EphA2 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-EphA2 (extracellular) Antibody (#AER-019), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region, showed immunoreactivity (green) in neuronal profiles in both soma (vertical arrows) and apical dendrites of pyramidal neurons (horizontal arrows). B. Pre-incubation of the antibody with EphA2 (extracellular) Blocking Peptide (#BLP-ER019), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, SO = stratum oriens, SR = stratum radiatum.
Expression of EphA2 in mouse cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-EphA2 (extracellular) Antibody (#AER-019), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. EphA2 immunoreactivity (green) appeared in neuronal profiles in both soma (vertical arrows) and apical dendrites of pyramidal neurons (horizontal arrows). B. Pre-incubation of the antibody with EphA2 (extracellular) Blocking Peptide (#BLP-ER019), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)HYLTAVGMGAKVELR, corresponding to amino acid residues 333 - 347 of human EphA2 (Accession P29317).
Accession (Uniprot) Number P29317
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
