Overview
Type: Synthetic peptide
Form: Lyophilized powder
EphA3 (extracellular) Blocking Peptide (#BLP-ER013) is the original antigen used for immunization during Anti-EphA3 (extracellular) Antibody (#AER-013) generation. The blocking peptide binds and ‘blocks’ Anti-EphA3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-EphA3 (extracellular) Antibody (#AER-013), (1:200).
3,4. Anti-EphA3 (extracellular) Antibody, preincubated with EphA3 (extracellular) Blocking Peptide (#BLP-ER013).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human K562 chronic myelogenous leukemia cell line lysate (lanes 2 and 6), human Malme-3M melanoma cell line lysate (lanes 3 and 7) and human HT-29 colorectal adenocarcinoma cell line lysate (lanes 4 and 8):1-4. Anti-EphA3 (extracellular) Antibody (#AER-013), (1:200).
5-8. Anti-EphA3 (extracellular) Antibody, preincubated with EphA3 (extracellular) Blocking Peptide (#BLP-ER013).
Expression of EphA3 in rat and mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-EphA3 (extracellular) Antibody (#AER-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. EphA3 staining (green) in rat hippocampal CA1 region is detected in the pyramidal layer (P). B. In mouse hippocampal CA1 region, EphA3 immunoreactivity (green) is observed in the pyramidal layer (P). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RARGTNVTISSLK, corresponding to amino acid residues 488-500 of human EphA3 (Accession P29320).
Accession (Uniprot) Number P29320
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
