Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)RARGTNVTISSLK, corresponding to amino acid residues 488-500 of human EphA3 (Accession P29320).
Accession (Uniprot) Number P29320
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-EphA3 (extracellular) Antibody (#AER-013), (1:200).
3,4. Anti-EphA3 (extracellular) Antibody, preincubated with EphA3 (extracellular) Blocking Peptide (#BLP-ER013).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human K562 chronic myelogenous leukemia cell line lysate (lanes 2 and 6), human Malme-3M melanoma cell line lysate (lanes 3 and 7) and human HT-29 colorectal adenocarcinoma cell line lysate (lanes 4 and 8):1-4. Anti-EphA3 (extracellular) Antibody (#AER-013), (1:200).
5-8. Anti-EphA3 (extracellular) Antibody, preincubated with EphA3 (extracellular) Blocking Peptide (#BLP-ER013).- Mouse and rat brain sections (1:200).
Last update: 02/01/2024
