Overview
Type: Synthetic peptide
Form: Lyophilized Powder
EphA4 (extracellular) Blocking Peptide (#BLP-ER044) is the original antigen used for immunization during Anti-EphA4 (extracellular) Antibody (#AER-044) generation. The blocking peptide binds and ‘blocks’ Anti-EphA4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-EphA4 (extracellular) Antibody (#AER-044), (1:200).
3-4. Anti-EphA4 (extracellular) Antibody, preincubated with EphA4 (extracellular) Blocking Peptide (BLP-ER044).
Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 5), human U-87 MG glioblastoma cell line lysate (lanes 2 and 6), human Jurkat T-cell leukemia cell line lysate (lanes 3 and 7) and human THP-1 monocytic leukemia cell line lysate (lanes 4 and 8):1-4. Anti-EphA4 (extracellular) Antibody (#AER-044), (1:200).
5-8. Anti-EphA4 (extracellular) Antibody, preincubated with EphA4 (extracellular) Blocking Peptide (BLP-ER044).
Properties
Sequence
- Peptide (C)ERSYRIVRTAARNTD, corresponding to amino acid residues 485 - 499 of human EPHA4 (Accession P54764).
Accession (Uniprot) Number Peptide (C)ERSYRIVRTAARNTD, corresponding to amino acid residues 485 - 499 of human EPHA4 (Accession P54764).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
