Overview
Type: Synthetic peptide
Form: Lyophilized powder
EphA6 (extracellular) Blocking Peptide (#BLP-ER016) is the original antigen used for immunization during Anti-EphA6 (extracellular) Antibody (#AER-016) generation. The blocking peptide binds and ‘blocks’ Anti-EphA6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1, 2. Anti-EphA6 (extracellular) Antibody (#AER-016), (1:200).
3, 4. Anti-EphA6 (extracellular) Antibody, preincubated with EphA6 (extracellular) Blocking Peptide (#BLP-ER016).
Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate:1. Anti-EphA6 (extracellular) Antibody (#AER-016), (1:400).
2. Anti-EphA6 (extracellular) Antibody, preincubated with EphA6 (extracellular) Blocking Peptide (#BLP-ER016).
Expression of EphA6 in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-EphA6 (extracellular) Antibody (#AER-016), (1:200), followed by goat anti-rabbit-AlexaFluor-488. EphA6 immunoreactivity (green) appears in pyramidal cells (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KEHEQLTYSSTRSK, corresponding to amino acid residues 482-495 of rat EphA6 (Accession P54758).
Accession (Uniprot) Number P54758
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
