Overview
Type: Synthetic peptide
Form: Lyophilized powder
Ephrin-A5 (extracellular) Blocking Peptide (#BLP-ER035) is the original antigen used for immunization during Anti-Ephrin-A5 (extracellular) Antibody (#AER-035) generation. The blocking peptide binds and ‘blocks’ Anti-Ephrin-A5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:1,2. Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200).
3,4. Anti-Ephrin-A5 (extracellular) Antibody, preincubated with Ephrin-A5 (extracellular) Blocking Peptide (#BLP-ER035).
Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate:1. Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200).
2. Anti-Ephrin-A5 (extracellular) Antibody, preincubated with Ephrin-A5 (extracellular) Blocking Peptide (#BLP-ER035).
Expression of Ephrin-A5 rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200), followed by goat anti-rabbit-AlexaFluor-488. Ephrin A5 staining (green) in hippocampal dentate gyrus is detected in hilar interneurons (arrows) and in the granule layer (GL). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DRVFDVNDKVENSLE, corresponding to amino acid residues 172-186 of human Ephrin-A5 (Accession P52803).
Accession (Uniprot) Number P52803
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
