Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Ephrin-B2 (extracellular) Blocking Peptide (#BLP-ER042) is the original antigen used for immunization during Anti-Ephrin-B2 (extracellular) Antibody (#AER-042) generation. The blocking peptide binds and ‘blocks’ Anti-Ephrin-B2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-Ephrin-B2 (extracellular) Antibody (#AER-042), (1:400).
3-4. Anti-Ephrin-B2 (extracellular) Antibody, preincubated with Ephrin-B2 (extracellular) Blocking Peptide (BLP-ER042).
Western blot analysis of human Colo-205 colon carcinoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4):1-2. Anti-Ephrin-B2 (extracellular) Antibody (#AER-042), (1:400).
3-4. Anti-Ephrin-B2 (extracellular) Antibody, preincubated with Ephrin-B2 (extracellular) Blocking Peptide (BLP-ER042).
Expression of Ephrin-B2 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Ephrin-B2 (extracellular) Antibody (#AER-042), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed immunoreactivity (green) in astrocytes of the hilus (H, arrows) and in neurons of the granule layer (G). B. Pre-incubation of the antibody with Ephrin-B2 (extracellular) Blocking Peptide (BLP-ER042), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)RRPELEAGTNGRS, corresponding to amino acid residues 181 - 193 of human Ephrin-B2 (Accession P52799).
Accession (Uniprot) Number P52799
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
