Cat #: AK-480_KIT
Size: 14 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-P2X1 Receptor (extracellular) Antibody Cat #: APR-022
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide CRPIYEFHGLYEEK, corresponding to amino acid residues 270-283 of human P2X1 receptor (Accession P51575). Extracellular loop.
Homology
Rat, mouse - 11/14 amino acid residues identical.
P2X1 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR022
Size:
40 µg
Anti-P2X2 Receptor (extracellular) Antibody Cat #: APR-025
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, LCI, WB
Reactivity:
M, R
Immunogen
Peptide (C)KSDYLKHSTFDQDS, corresponding to amino acid residues 207-220 of rat P2X2 with replacement of cysteine 214 (C214) with serine (*S) (Accession P49653). Extracellular.
Homology
Rat, mouse - 13/14 amino acid residues identical.
Will not recognize human P2X2.
Will not recognize human P2X2.
P2X2 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR025
Size:
40 µg
Anti-P2X3 Receptor (extracellular) Antibody Cat #: APR-026
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
M, R
Immunogen
Peptide (C)KGFGRYANRVMDVSD, corresponding to amino acid residues 65-79 of rat P2X3 receptor (Accession P49654). Extracellular.
Homology
Mouse – identical; human - 13/15 amino acid residues identical.
P2X3 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR026
Size:
40 µg
Anti-P2X4 Receptor (extracellular) Antibody Cat #: APR-024
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)RDLAGKEQRTLTK, corresponding to amino acid residues 301-313 of rat P2X4 receptor (Accession P51577). Extracellular.
Homology
Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.
P2X4 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR024
Size:
40 µg
Anti-P2X5 Receptor (extracellular) Antibody Cat #: APR-027
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IFC, LCI, WB
Reactivity:
M, R
Immunogen
Peptide (C)HRSHTRLAEMKYE, corresponding to amino acid residues 299-311 of rat P2X5 receptor (Accession P51578).
Homology
Mouse –12/13 amino acid residues; human – 10/13 amino acid residues.
P2X5 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR027
Size:
40 µg
Anti-P2X6 Receptor (extracellular) Antibody Cat #: APR-028
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
WB
Reactivity:
M, R
Immunogen
Peptide CPEGEMGTYSHGIK, corresponding to amino acid residues 136-149 of rat P2X6 receptor (Accession P51579). Extracellular.
Homology
Mouse - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical.
P2X6 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR028
Size:
40 µg
Anti-P2X7 Receptor (extracellular) Antibody Cat #: APR-008
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.
Homology
Human, rat - identical; bovine - 14/17 amino acid residues identical.
P2X7 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR008
Size:
40 µg
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For research purposes only, not for human use
