Overview
Alternative Name Fasciculin-2, Fas-2, Fas2, FAS-II, Toxin TA1, Acetylcholinesterase toxin F-VII, fasciculin, fasciculin II, fasciculin 2
Lyophilized Powder yes
Origin Natural peptide isolated from Dendroaspis angusticeps (Eastern green mamba).
MW: 6749 Da
Purity: >99% (HPLC)
Effective concentration 1-10 nM.
Sequence TMCYSHTTTSRAILTNCGENSCYRKSRRHPPKMVLGRGCGCPPGDDNLEVKCCTSPDKCNY.
Modifications Disulfide bonds between Cys3-Cys22, Cys17-Cys39, Cys41-Cys52 and Cys53-Cys59.
Molecular formula C276H438N88O90S10.
CAS No.: 95506-56-2
Activity Fasciculin-II selectively and potently binds and inhibits acetylcholinesterase.
References-Activity
- Anglister, L. et al. (1978) J. Mol. Biol. 125, 293.
- Ellman, G.L. et al. (1961) Biochem. Pharmacol. 7, 88.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any conventional buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to two weeks at 4°C or six months at -20°C.
Our bioassay
Alomone Labs Fasciculin-II potently inhibits Acetylcholinesterase (AChE) activity.AChE activity was determined colorimetrically using Ellman's reagent, as production of thiocholine from acetylthiocholine. Absorbance was measured at 405 nm after a 30 min incubation. Normalized activity of 400 mU/ml (black) human AChE, as percent of control, was plotted against increasing concentrations of Fasciculin-II (#F-225), showing concentration-dependent inhibition with pIC50 ≈ 9.
Scientific background
Fasciculin-II is a 61 amino acid peptidyl toxin with four disulfide bonds, isolated from the Eastern green mamba (Dendroaspis angusticeps) venom. Fasciculin-II belongs to the three finger protein of Elapidae toxin family. Fasciculin-II differs from Fasciculin-I only at one position by a replacement of Tyr with Asp or Asn1.
Fasciculin-II was shown to be a selective potent and reversible blocker of acetylcholinesterase by binding to the peripheral site of the enzyme1. Injection of Fasciculin-II to mice causes severe, generalized, and long-lasting twitching (fasciculation) due to its ability to prevent degradation and therefore promote acetylcholine accumulation at the neuromuscular junction2.
Peptide Content: 100%
Lyophilized Powder
For research purposes only, not for human use
Last Update: 02/01/2024
Specifications
Citations
Citations
