Overview
Type: Synthetic peptide
Form: Lyophilized Powder
FLRT3 (extracellular) Blocking Peptide (#BLP-FR053) is the original antigen used for immunization during Anti-FLRT3 (extracellular) Antibody (#AFR-053) generation. The blocking peptide binds and ‘blocks’ Anti-FLRT3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Anti-FLRT3 (extracellular) Antibody (#AFR-053), (1:200).
3-4. Anti-FLRT3 (extracellular) Antibody, preincubated with FLRT3 (extracellular) Blocking Peptide (#BLP-FR053).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 4):1-2. Anti-FLRT3 (extracellular) Antibody (#AFR-053), (1:200).
3-4. Anti-FLRT3 (extracellular) Antibody, preincubated with FLRT3 (extracellular) Blocking Peptide (#BLP-FR053).
Expression of FLRT3 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-FLRT3 (extracellular) Antibody (#AFR-053), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed immunoreactivity (green) in the granule layer (G) and in the hilus (H, arrows). B. Pre-incubation of the antibody with FLRT3 (extracellular) Blocking Peptide (#BLP-FR053), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of FLRT3 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-FLRT3 (extracellular) Antibody (#AFR-053), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. FLRT3 immunoreactivity (green) appears in the purkinje cell layer (P, arrows). B. Pre-incubation of the antibody with FLRT3 (extracellular) Blocking Peptide (#BLP-FR053), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide ((C)KQPDIKNPKLTKDQR, corresponding to amino acid residues 387 - 401 of rat FLRT3 (Accession B1H234).
Accession (Uniprot) Number B1H234
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
