Overview
Type: Synthetic peptide
Form: Lyophilized powder
Frizzled-1/FZD1 (extracellular) Blocking Peptide (#BLP-FR041) is the original antigen used for immunization during Anti-Frizzled-1 (FZD1) (extracellular) Antibody (#AFR-041) generation. The blocking peptide binds and ‘blocks’ Anti-Frizzled-1/FZD1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) kidney lysates:1,2. Anti-Frizzled-1 (FZD1) (extracellular) Antibody (#AFR-041), (1:200).
3,4. Anti-Frizzled-1 (FZD1) (extracellular) Antibody, preincubated with Frizzled-1/FZD1 (extracellular) Blocking Peptide (#BLP-FR041).
Properties
Sequence
- (C)PEFWTSNPQHGGG, corresponding to amino acid residues 238-250 of rat Frizzled-1 (Accession Q08463).
Accession (Uniprot) Number Q08463
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
