Overview
Type: Synthetic peptide
Form: Lyophilized powder
FSHR (extracellular) Blocking Peptide (#BLP-FR010) is the original antigen used for immunization during Anti-FSHR (extracellular) Antibody (#AFR-010) generation. The blocking peptide binds and ‘blocks’ Anti-FSHR (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat ovary (lane 1 and 4), rat testis (lane 2 and 5) and human OVCAR3 cell line (lane 3 and 6) lysates:1-3. Anti-FSHR (extracellular) Antibody (extracellular) Antibody (#AFR-010), (1:200).
4-6. Anti-FSHR (extracellular) Antibody, preincubated with FSHR (extracellular) Blocking Peptide (#BLP-FR010).
Expression of FSHR in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-FSHR (extracellular) Antibody (#AFR-010), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat ventromedial hypothalamus region, showed immunoreactivity (green) in processes (arrows) in the periventricular hypothalamus. B. Pre-incubation of the antibody with FSHR (extracellular) Blocking Peptide (#BLP-FR010), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V= Third ventricle.
Expression of FSHR in mouse cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-FSHR (extracellular) Antibody (#AFR-010), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. FSHR immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with FSHR (extracellular) Blocking Peptide (#BLP-FR010), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)SNTGIKHLPAVHK, corresponding to amino acid residues 128-140 of rat FSHR (Accession P20395).
Accession (Uniprot) Number P20395
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
