Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA Transporter 2/GAT-2 Blocking Peptide (#BLP-GT002) is the original antigen used for immunization during Anti-GABA Transporter 2 (GAT-2) Antibody (#AGT-002) generation. The blocking peptide binds and ‘blocks’ Anti-GABA Transporter 2/GAT-2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-GABA Transporter 2 (GAT-2) Antibody (#AGT-002), (1:200).
3,4. Anti-GABA Transporter 2 (GAT-2) Antibody, preincubated with GABA Transporter 2/GAT-2 Blocking Peptide (#BLP-GT002).
Expression of GABA transporter 2 in rat lungImmunohistochemical staining of rat lung paraffin-embedded sections using Anti-GABA Transporter 2 (GAT-2) Antibody (#AGT-002), (1:100). GAT-2 (brown) is expressed in the respiratory epithelium of the bronchioli. Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)KVEEDGTLEREQWTNK, corresponding to amino acids residues 23-38 of rat GAT-2 (Accession P31646).
Accession (Uniprot) Number P31646
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
