Name: GABA(A) α1 Receptor (extracellular) Blocking Peptide
Brand: Alomone Labs
SKU: BLP-GA001

Overview

Cat #: BLP-GA001

Type: Synthetic peptide

Form: Lyophilized powder

GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001) is the original antigen used for immunization during Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) α1 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Certificate of Analysis SDS

Applications: wb, ihc

Application key:

WB- Western blot, IHC- Immunohistochemistry

For research purposes only. not for human use

Applications

Demonstration of Pre-adsorption control

Western blot analysis of rat brain lysates:
1. Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001), (1:1000).
2. Anti-GABA (A) α1 Receptor (extracellular) Antibody, preincubated with GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001).
Expression of GABRA1 in mouse hippocampus
Immunohistochemical staining of mouse hippocampus using Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001). A. Distribution of GABRA1 (red). B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that distribution of GABRA1 is restricted to neurons and their processes. DAPI is used as the counterstain (blue).
Colocalization of Ca_V1.2 and GABA(A) α1 Receptor in rat cerebellum
Immunohistochemical staining of rat cerebellum using Guinea pig Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003-GP) and Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001). A. Ca_V1.2 (green) is detected in the granule layer of the cerebellum (G) and in the upper molecular layer (star). B. In the same section, GABRA1 (red) is seen in the granule layer. C. Merge of the two images reveals high degree of colocalization between Ca_V1.2 and GABRA1 in the granule layer.
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) membranes and human brain neuroblastoma (SH-SY5Y) (lanes 3 and 6) cell lysate:
1-3. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP), (1:400).
4-6. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody, preincubated with GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001).

Following a broad screen of secondary antibodies, the following were used for this application:
Western blot analysis:
#106-035-006 (Jackson ImmunoResearch)
#A7289 (Sigma)
Multiplex staining of KCC2 and GABA(A) α1 Receptor in rat cerebellum
Immunohistochemical staining of perfusion-fixed frozen rat brain sections using rabbit Anti-KCC2 (SLC12A5) Antibody (#AKT-005), (1:400) and Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP), (1:400). A. KCC2 immunoreactivity (green) is detected in both the granule layer (GL) and the molecular layer (MOL). B. GABA(A) α1 receptor staining (red) is observed mainly in the granule layer (GL). C. Merge of the two images demonstrates colocalization in the granule layer (arrows). Cell nuclei are stained with DAPI (blue).
Expression of GABA(A) α1 Receptor in rat hippocampus
Immunohistochemical staining of rat hippocampal dentate gyrus sections using Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP). A. GABRA1 staining (red) appears in neuronal soma (horizontal arrows in panel B) and in the neuronal processes (vertical arrows in panel B). B. GABRA1 staining merged with DAPI counterstain (blue).

Properties

Sequence

QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABRA1 (Accession P62813).

Accession (Uniprot) Number P62813

Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.

Purity >70%

Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.

Reconstitution 100 µl double distilled water (DDW).

Storage After Reconstitution -20°C.

Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.

Standard Quality Control Of Each Lot Western blot analysis.

Specific Use it with:

Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry,immunocytochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is highly suited to detect GABRA1 in live cells. It has been designed to recognize GABRA1 from human, rat, and mouse samples.

Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001)
Alomone Labs offers a highly specific antibody directed against an extracellular epitope of rat GABA (A) α1 receptor. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP, formerly AGP-083) raised in guinea pig can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is highly suited to detect GABRA1 in live cells. It has been designed to recognize GABRA1 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP)