Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABRA1 (Accession P62813).
Accession (Uniprot) Number P62813
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates:1. Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001), (1:1000).
2. Anti-GABA (A) α1 Receptor (extracellular) Antibody, preincubated with GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001).- Mouse and rat hippocampus.
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) membranes and human brain neuroblastoma (SH-SY5Y) (lanes 3 and 6) cell lysate:1-3. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP), (1:400).
4-6. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody, preincubated with GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001).Following a broad screen of secondary antibodies, the following were used for this application:
Western blot analysis:
#106-035-006 (Jackson ImmunoResearch)
#A7289 (Sigma)- Rat brain sections.
Last update: 02/01/2024
