Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA(A) α3 Receptor (extracellular) Blocking Peptide (#BLP-GA003) is the original antigen used for immunization during Anti-GABA(A) α3 Receptor (extracellular) Antibody (#AGA-003) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) α3 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-GABA(A) α3 Receptor (extracellular) Antibody (#AGA-003), (1:200).
2. Anti-GABA(A) α3 Receptor (extracellular) Antibody preincubated with GABA(A) α3 Receptor (extracellular) Blocking Peptide (#BLP-GA003).
Expression of GABRA3 in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-GABA(A) α3 Receptor (extracellular) Antibody (#AGA-003), (green), (1:100). GABRA3 is localized to the molecular layer (asterisk) and a portion of the Purkinje cell body (arrows), which is outlined by axonal staining using mouse anti-neurofilament 200 (red).
Properties
Sequence
- QGESRRQEPGDFVKQ(C), corresponding to amino acid residues 29-43 of human GABRA3 (Accession P34903).
Accession (Uniprot) Number P34903
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
