Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA(A) α4 Receptor (extracellular) Blocking Peptide (#BLP-GA008) is the original antigen used for immunization during Anti-GABA(A) α4 Receptor (extracellular) Antibody (#AGA-008) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) α4 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and human CCF-STGI Brain astrocytoma cell line lysate (lanes 3 and 6):1-3. Anti-GABA(A) α4 Receptor (extracellular) Antibody (#AGA-008), (1:200).
4-6. Anti-GABA(A) α4 Receptor (extracellular) Antibody, preincubated with GABA(A) α4 Receptor (extracellular) Blocking Peptide (#BLP-GA008).
Expression of GABA(A) α4 Receptor in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-GABA(A) α4 Receptor (extracellular) Antibody (#AGA-008). A. GABRA4 staining (green) appears in the molecular layer (Mol) and around the soma of Purkinje cells (arrow). B. Parvalbumin (red), a marker of Purkinje and interneuronal cells, is stained in the same section. C. Merge of the images demonstrates expression of GABRA4 around the soma of Purkinje cells. DAPI is used as the counterstain (blue).
Properties
Sequence
- NSKDEKLSPENFTR(C), corresponding to amino acid residues 37-50 of rat GABRA4 with replacement of C44 with serine (Accession P28471).
Accession (Uniprot) Number P28471
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
