Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA(A) α5 Receptor Blocking Peptide (#BLP-GA025) is the original antigen used for immunization during Anti-GABA(A) α5 Receptor Antibody (#AGA-025) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) α5 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat hippocampus (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:1,2. Anti-GABA(A) α5 Receptor Antibody (#AGA-025), (1:400).
3,4. Anti-GABA(A) α5 Receptor Antibody, preincubated with GABA(A) α5 Receptor Blocking Peptide (#BLP-GA025).
Expression of GABRA5 in mouse brainImmunohistochemical staining of mouse hippocampus sections using Anti-GABA(A) α5 Receptor Antibody (#AGA-025), (1;600). A. GABRA5 staining (green). B. Merged image of GABRA5 (green) with nuclei staining using DAPI as the counterstain (blue). GABRA5 is detected in the pyramidal layer (Pyr) (horizontal arrows) and in some interneurons (vertical arrow).
Properties
Sequence
- (C)KKERELILNKSTN, corresponding to amino acid residues 364-376 of rat GABRA5 (Accession P19969).
Accession (Uniprot) Number P19969
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
