Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA(A) α6 Receptor (extracellular) Blocking Peptide (#BLP-GA004) is the original antigen used for immunization during Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) α6 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-GABA(A) α6 Receptor (extracellular) Antibody, (1:200).
2. Anti-GABA(A) α6 Receptor (extracellular) Antibody, preincubated with GABA(A) α6 Receptor (extracellular) Blocking Peptide (#BLP-GA004).
Expression of GABRA6 in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004). A. GABRA6 (red) appears in the granule layer (asterisk). B. Parvalbumin (green) appears in Purkinje cells and in the molecular layer. C. Merge of GABRA6 and parvalbumin indicates that GABRA6 is restricted to granule cells. DAPI is used as the counterstain (blue).
Properties
Sequence
- QLEDEGNFYSENVSRILDN(C), corresponding to amino acid residues 20-37 of rat GABRA6 (Accession P30191).
Accession (Uniprot) Number P30191
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
