Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GABA(A) β2 Receptor Blocking Peptide (#BLP-GA012) is the original antigen used for immunization during Anti-GABA(A) β2 Receptor Antibody (#AGA-012) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) β2 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-GABA(A) β2 Receptor Antibody (#AGA-012), (1:200).
3-4. Anti-GABA(A) β2 Receptor Antibody, preincubated with GABA(A) β2 Receptor Blocking Peptide (BLP-GA012).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4):1-2. Anti-GABA(A) β2 Receptor Antibody (#AGA-012), (1:200).
3-4. Anti-GABA(A) β2 Receptor Antibody, preincubated with GABA(A) β2 Receptor Blocking Peptide (BLP-GA012).
Expression of GABA(A) Receptor Beta 2 in mouse hypothalamus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections Anti-GABA(A) β2 Receptor Antibody (#AGA-012), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the periventricular hypothalamus region, showed GABA(A) Receptor Beta 2 immunoreactivity (green) in periventricular glial processes (arrows). B. Pre-incubation of the antibody with GABA(A) β2 Receptor Blocking Peptide (BLP-GA012), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = 3rd ventricle.
Expression of GABA(A) Receptor Beta 2 in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-GABA(A) β2 Receptor Antibody (#AGA-012), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the periventricular hypothalamus region, showed GABA(A) Receptor Beta 2 immunoreactivity (green) in periventricular glial processes (arrows). B. Pre-incubation of the antibody with GABA(A) β2 Receptor Blocking Peptide (BLP-GA012), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = 3rd ventricle.
Properties
Sequence
- Peptide (C)KAGLPRHSFGRNALE, corresponding to amino acid residues 405 - 419 of rat GABRB2 (Accession P63138).
Accession (Uniprot) Number P63138
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
