Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA(A) γ1 Receptor Blocking Peptide (#BLP-GA016) is the original antigen used for immunization during Anti-GABA(A) γ1 Receptor Antibody (#AGA-016) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) γ1 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-GABA(A) γ1 Receptor Antibody (#AGA-016), (1:500).
3,4. Anti-GABA(A) γ1 Receptor Antibody, preincubated with GABA(A) γ1 Receptor Blocking Peptide (#BLP-GA016).
Expression of GABA(A) γ1 receptor in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-GABA(A) γ1 Receptor Antibody (#AGA-016), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Staining (red) appears in neuronal cell bodies. B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
Properties
Sequence
- (C)HAGSTLIPMNNISMPQGE, corresponding to amino acid residues 382-399 of rat GABA(A) γ1 receptor (Accession P23574).
Accession (Uniprot) Number P23574
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
