Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA(A) δ Receptor (extracellular) Blocking Peptide (#BLP-GA014) is the original antigen used for immunization during Anti-GABA(A) δ Receptor (extracellular) Antibody (#AGA-014) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) δ Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), rat cerebellum (lanes 2 and 5) and mouse brain (lanes 3 and 6):1-3. Anti-GABA(A) δ Receptor (extracellular) Antibody (#AGA-014), (1:200).
4-6. Anti-GABA(A) δ Receptor (extracellular) Antibody, preincubated with GABA(A) δ Receptor (extracellular) Blocking Peptide (#BLP-GA014).
Expression of GABA(A) δ receptor in rat cerebellumImmunohistochemical staining of GABA(A) δ receptor in rat cerebellum using Anti-GABA(A) δ Receptor (extracellular) Antibody (#AGA-014). GABA(A) δ receptor (red) appears exclusively in the granule layer (G). Above and below the granule layer is the molecular layer (M). DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)HHGARAMNDIGDYVGSN, corresponding to amino acid residues 19-35 of rat GABA(A) δ receptor (Accession P18506).
Accession (Uniprot) Number P18506
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
