Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA(A) θ Receptor/GABRQ (extracellular) Blocking Peptide (#BLP-GA018) is the original antigen used for immunization during Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) θ Receptor/GABRQ (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and Human CCF-STGI brain astrocytoma cell lysate (lanes 3 and 6):1-3. Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018), (1:500).
4-6. Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody, preincubated with GABA(A) θ Receptor/GABRQ (extracellular) Blocking Peptide (#BLP-GA018).
Expression of of GABA(A) θ receptor in rat hypothalamusImmunohistochemical staining of rat hypothalamus using Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018). A. GABRQ staining (red) is detected in the mammillary nucleus which is part of the posterior hypothalamus (arrows demarcate nucleus border). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge images of A and B.
Properties
Sequence
- DYRMHEKLWVPDC, corresponding to amino acid residues 131-143 of mouse GABRQ (Accession Q9JLF1).
Accession (Uniprot) Number Q9JLF1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
