Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA(A) π Receptor (extracellular) Blocking Peptide (#BLP-GA019) is the original antigen used for immunization during Anti-GABA(A) π Receptor (extracellular) Antibody (#AGA-019) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) π Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:1, 2. Anti-GABA(A) π Receptor/GABRP (extracellular) Antibody (#AGA-019), (1:200).
3, 4. Anti-GABA(A) π Receptor/GABRP (extracellular) Antibody, preincubated with GABA(A) π Receptor (extracellular) Blocking Peptide (#BLP-GA019).
Expression of GABA(A) π Receptor in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GABA(A) π Receptor/GABRP (extracellular) Antibody (#AGA-019), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal dentate gyrus (DG) region showed GABARP immunoreactivity (green) in hilar (H) neurons (vertical arrows). B. Pre-incubation of the antibody with GABA(A) π Receptor/GABRP (extracellular) Blocking Peptide (BLP-GA019), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DGNDVEFSWLRGND, corresponding to amino acid residues 184-197 of rat GABA(A) Pi Receptor (Accession O09028).
Accession (Uniprot) Number O09028
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
