Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABA(B) R1 (extracellular) Blocking Peptide (#BLP-GB001) is the original antigen used for immunization during Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(B) R1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001), (1:200).
2. Anti-GABA(B) R1 (extracellular) Antibody, preincubated with GABA(B) R1 (extracellular) Blocking Peptide (#BLP-GB001).
Expression of GABA(B) receptor 1 in mouse hippocampusImmunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001), (1:100). A. GABBR1 staining (green) is detected in neurons in the CA3 field and in the dentate granule layer (short arrows), as well as in dendrites of CA3 pyramidal neurons (long arrows). B. Staining with mouse anti-GAP43 antibody (red) sets apart the stratum lacunosum moleculare (SLM). C. Confocal merge suggests the presence of GABBR1 in pyramidal neurons.
Properties
Sequence
- CRGEREVVGPKVRK, corresponding to amino acid residues 66-79 of rat GABBR1 (Accession Q9Z0U4).
Accession (Uniprot) Number Q9Z0U4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
