Overview
Type: Synthetic peptide
Form: Lyophilized powder
GABARAP Blocking Peptide (#BLP-IP016) is the original antigen used for immunization during Anti-GABARAP Antibody (#AIP-016) generation. The blocking peptide binds and ‘blocks’ Anti-GABARAP primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and human neuroblastoma (SH-SY5Y) cell line (lanes 3 and 6) lysates:1-3. Anti-GABARAP Antibody (#AIP-016), (1:800).
4-6. Anti-GABARAP Antibody, preincubated with GABARAP Blocking Peptide (#BLP-IP016).
Expression of GABARAP in rat cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GABARAP Antibody (#AIP-016), (1:1000), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. GABARAP immunoreactivity (red) appears in cortical neurons (arrows). B. Pre-incubation of the antibody with GABARAP Blocking Peptide (#BLP-IP016), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KFVYKEEHPFEKRR, corresponding to amino acid residues 2-15 of rat GABARAP (Accession P60517).
Accession (Uniprot) Number P60517
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
