Overview
Type: Synthetic peptide
Form: Lyophilized powder
GALR3 (extracellular) Blocking Peptide (#BLP-GR013) is the original antigen used for immunization during Anti-GALR3 (extracellular) Antibody (#AGR-013) generation. The blocking peptide binds and ‘blocks’ Anti-GALR3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane:1. Anti-GALR3 (extracellular) Antibody (#AGR-013), (1:200).
2. Anti-GALR3 (extracellular) Antibody, preincubated with GALR3 (extracellular) Blocking Peptide (#BLP-GR013).
Expression of Galanin receptor 3 in rat hippocampusImmunohistochemical staining of rat hippocampus using Anti-GALR3 (extracellular) Antibody (#AGR-013). A. Galanin receptor 3 (green) appears in apical dendrites of pyramidal neurons in CA1 (arrows). B. MAP2 (red), a marker of axons and dendrites, is stained in the same section. GALR3 appears to be expressed in a subset of apical dendrites (arrows). DAPI is used as the counterstain (blue).
Properties
Sequence
- SYYGTVRYGALELC, corresponding to amino acid residues 159-172 of mouse Galanin receptor type 3 (Accession O88853).
Accession (Uniprot) Number O88853
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
