Overview
Type: Synthetic peptide
Form: Lyophilized powder
GAP43 Blocking Peptide (#BLP-NR016) is the original antigen used for immunization during Anti-GAP43 Antibody (#ANR-016) generation. The blocking peptide binds and ‘blocks’ Anti-GAP43 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2 Anti-GAP43 Antibody (#ANR-016), (1:400).
3,4. Anti-GAP43 Antibody, preincubated with GAP43 Blocking Peptide (#BLP-NR016).
Expression of GAP43 in rat hippocampusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GAP43 Antibody (#ANR-016), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. GAP43 staining (green) appears in a layer bordering granule layer (G), (arrow). B. Calbindin D28k staining (red) is detected in granule layer (G) neurons. C. Merge of the two images demonstrates distinct spatial localizations in the hippocampal dentate gyrus. Cell nuclei are stained with DAPI (blue).
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1, 2. Guinea Pig Anti-GAP43 Antibody (#ANR-016-GP), (1:400).
3, 4. Guinea Pig Anti-GAP43 Antibody, preincubated with GAP43 Blocking Peptide (#BLP-NR016).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Guinea Pig Anti-GAP43 Antibody (#ANR-016-GP), (1:400).
2. Guinea Pig Anti-GAP43 Antibody, preincubated with GAP43 Blocking Peptide (#BLP-NR016).
Expression of GAP43 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-GAP43 Antibody (#ANR-016-GP), (1:300), followed by goat anti-guinea pig - Alexa Fluor-594. A. Staining in the hippocampal dentate gyrus region, showed immunoreactivity (red) in the subgranular layer (arrows), over the granule layer (G) and over the outer molecular layer (OML). B. Pre-incubation of the antibody with GAP43 Blocking Peptide (#BLP-NR016), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KPKESARQDEGKED, corresponding to amino acid residues 205-218 of rat GAP43 (Accession P07936).
Accession (Uniprot) Number P07936
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
