Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)DDNLVYHILRKHSAKR, corresponding to amino acid residues 192-207 of human GDNF (precursor) (Accession P39905).
Accession (Uniprot) Number P39905
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of Recombinant human GDNF protein:Lanes 1, 4: 50 ng Recombinant human GDNF protein (#G-240).
Lanes 2, 5: 10 ng Recombinant human GDNF protein.
Lanes 3, 4: 1 ng Recombinant human GDNF protein.
Lanes 1-3: Anti-GDNF Antibody (#ANT-014), (1:1000).
Lanes 4-5: Anti-GDNF Antibody, preincubated with GDNF Blocking Peptide (#BLP-NT014).- Rat dorsal root ganglion sections (frozen) (1:100).
Last update: 02/01/2024
