Overview
Lyophilized Powder yes
Origin Glycine max (Soy bean).
Source Natural
MW: 270.2
Purity: >99%
Effective concentration 0.5-10 µM.
Structure
Chemical name 5,7-Dihydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one.
Molecular formula C15H10O5.
CAS No.: 446-72-0
Activity Genistein, apart from a broad range of biological roles, is well established as a tyrosine kinase inhibitor1.
References-Activity
- Akiyama, T. et al. (1987) J. Biol. Chem. 262, 5592.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to one week at 4°C or three months at -20°C.
Protect from light.
Protect from light.
Our bioassay
Alomone Labs Genistein inhibits tyrosine kinase in HeLa cells.Cells were grown to 70% confluence, and then starved for 18 h. The cells were preincubated for 30 min in the presence of 0.5 µM Genistein (#G-300) and then stimulated for 2 h with 20% fetal calf serum or culture medium. The cell extracts were blotted and probed with phospho-Tyr specific antibodies.
References - Scientific background
- Polkowski, K. and Mazurek, A.P. (2000) Acta Pol. Pharm. 57, 135.
- Sarkar, F.H. and Li, Y. (2002) Cancer Metastasis Rev. 21, 265.
- Salti, G.I. (2000) Eur. J. Cancer. 36, 796.
- Spinozzi, F. et al. (1994) Leuk. Res. 18, 431.
- Myoung, H. et al. (2003) Cancer Sci. 94, 215.
- Li, Y. and Sarkar, F.H. (2002) Cancer Lett. 186, 157.
- Huang, R.Q. et al. (1999) Brain Res. Mol. Brain Res. 67, 177.
- Constantinou, A. and Huberman, E. (1995) Proc. Soc. Exp. Biol. Med. 208, 109.
- Khoshyomn, S. et al. (2002) J. Neurooncol. 57, 193.
- Tamura, S. et al. (2003) Pigment Cell Res. 16, 470.
Scientific background An isoflavonoid derived from soy products,1 Genistein inhibits protein tyrosine kinases including the EGF receptor kinase (IC50 = 2.6 µM). The inhibition is competitive with respect to ATP and non-competitive with respect to the phosphate acceptor.1,2 It has a trivial effect on the activity of PKA and PKC (IC50 > 350 µM).1,2 In addition, Genistein inhibits Topoisomerase II activity,3 causes cell cycle arrest and apoptosis in Jurkat T-leukemia cells.4 It down regulates the transcription of genes involved in angiogenesis,5,6 and inhibits the action of GABA on recombinant GABA-A receptors.7 Genistein also inhibits tumor cell proliferation and induces tumor cell differentiation.8-10
Lyophilized Powder
Genistein (#G-300) is a highly pure, natural, and biologically active compound.
For research purposes only, not for human use
Last Update: 07/05/2024
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