Overview
Type: Synthetic peptide
Form: Lyophilized powder
Gephyrin Blocking Peptide (#BLP-IP005) is the original antigen used for immunization during Anti-Gephyrin Antibody (#AIP-005) generation. The blocking peptide binds and ‘blocks’ Anti-Gephyrin primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-Gephyrin Antibody (#AIP-005), (1:200).
3,4. Anti-Gephyrin Antibody, preincubated with Gephyrin Blocking Peptide (#BLP-IP005).
Expression of Gephyrin in rat hippocampusImmunohistochemical staining of rat hippocampal dentate gyrus sections using Anti-Gephyrin Antibody (#AIP-005). A. Gephyrin staining (green) appears in hilus of the dentate gyrus (arrows). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge image of panel A and B.
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-Gephyrin Antibody (#AIP-005-GP), (1:200).
3-4. Guinea Pig Anti-Gephyrin Antibody (#AIP-005-GP), preincubated with Gephyrin Blocking Peptide (#BLP-IP005).
Expression of Gephyrin in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-Gephyrin Antibody (#AIP-005-GP), (1:1000), followed by donkey anti-guinea pig-biotin and Streptavidin-Cy3. A. Gephyrin showed immunoreactivity (red) in the mossy fiber terminal zone (MFT, arrow) and in the pyramidal layer of the CA3 region (CA3). B. Pre-incubation of the antibody with Gephyrin Blocking Peptide (#BLP-IP005), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RDAIVKVKEVHDE, corresponding to amino acid residues 183-195 of rat Gephyrin (Accession Q03555).
Accession (Uniprot) Number Q03555
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
