Cat #: BLP-IP005
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)RDAIVKVKEVHDE, corresponding to amino acid residues 183-195 of rat Gephyrin (Accession Q03555).
Accession (Uniprot) Number Q03555
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-Gephyrin Antibody (#AIP-005), (1:200).
3,4. Anti-Gephyrin Antibody, preincubated with Gephyrin Blocking Peptide (#BLP-IP005). - Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-Gephyrin Antibody (#AIP-005-GP), (1:200).
3-4. Guinea Pig Anti-Gephyrin Antibody (#AIP-005-GP), preincubated with Gephyrin Blocking Peptide (#BLP-IP005).
Last update: 02/01/2024