Cat #: BLP-FP001
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)RHLGTIPRLSLSR, corresponding to amino acid residues 27-39 of rat Glial fibrillary acidic protein (Accession P47819).
Accession (Uniprot) Number P47819
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain membranes (lanes 1 and 3), (1:2000) and mouse brain lysate (lanes 2 and 4), (1:400):1,2. Anti-GFAP Antibody (#AFP-001).
3,4. Anti-GFAP Antibody, preincubated with GFAP Blocking Peptide (#BLP-FP001). - Rat brain sections (1:2000).
- Western blot analysis of rat brain synaptosome lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Guinea Pig Anti-GFAP Antibody (#AFP-001-GP), (1:500).
3-4. Guinea Pig Anti-GFAP Antibody, preincubated with GFAP Blocking Peptide (BLP-FP001).
Last update: 12/05/2024