Overview
Type: Synthetic peptide
Form: Lyophilized powder
GFR α 1 (extracellular) Blocking Peptide (#BLP-NT021) is the original antigen used for immunization during Anti-GFR alpha 1 (extracellular) Antibody (#ANT-021) generation. The blocking peptide binds and ‘blocks’ Anti-GFR α 1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-GFR alpha 1 (extracellular) Antibody (#ANT-021), (1:200).
3,4. Anti-GFR alpha 1 (extracellular) Antibody, preincubated with GFR α 1 (extracellular) Blocking Peptide (#BLP-NT021).
Expression of GFRA1 in rat neocortexImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GFR alpha 1 (extracellular) Antibody (#ANT-021), (1:100). A. GFRA1 (green) is visualized in neocortex neurons. B. Neurons expressing γ-amino butyric acid (GABA) are labeled with parvalbumin (red). C. Merge of the two images demonstrates partial colocalization (arrows).
Properties
Sequence
- CRSAMEALKQKS, corresponding to amino acid residues 72-83 of rat GFRA1 (Accession Q62997).
Accession (Uniprot) Number Q62997
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
