Cat #: BLP-NT024
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)GSRDG(S)PEEGGPR, corresponding to amino acid residues 160-172 of rat GFRA4 (Accession Q9EPI2).
Accession (Uniprot) Number Q9EPI2
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-GFR alpha 4 (extracellular) Antibody (#ANT-024), (1:800).
3,4. Anti-GFR alpha 4 (extracellular) Antibody, preincubated with GFR α 4 (extracellular) Blocking Peptide (#BLP-NT024). - Rat brain free-floating frozen sections (1:100).
Last update: 02/01/2024