Overview
Type: Synthetic peptide
Form: Lyophilized powder
GIPC1 Blocking Peptide (#BLP-PZ045) is the original antigen used for immunization during Anti-GIPC1 Antibody (#APZ-045) generation. The blocking peptide binds and ‘blocks’ Anti-GIPC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1,2. Anti-GIPC1 Antibody (#APZ-045), (1:400).
3,4. Anti-GIPC1 Antibody, preincubated with GIPC1 Blocking Peptide (#BLP-PZ045).
Western blot analysis of human U87-MG glioblastoma cell line lysate:1. Anti-GIPC1 Antibody (#APZ-045), (1:400).
2. Anti-GIPC1 Antibody, preincubated with GIPC1 Blocking Peptide (#BLP-PZ045).
Expression of GIPC1 in mouse and rat hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections with Anti-GIPC1 Antibody (#APZ-045), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. In mouse brain GIPC1 staining (green) appears in neurons of the pyramidal layer (P) and their apical dendrites (arrows). B. In rat brain similar staining is observed. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KAPPLVENEEAEPGR, corresponding to amino acid residues 10-24 of human GIPC1 (Accession O14908).
Accession (Uniprot) Number O14908
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
