Overview
Type: Synthetic peptide
Form: Lyophilized powder
GIPR (extracellular) Blocking Peptide (#BLP-GR025) is the original antigen used for immunization during Anti-GIPR (extracellular) Antibody (#AGR-025) generation. The blocking peptide binds and ‘blocks’ Anti-GIPR (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat pancreas membranes (lanes 3 and 7) and mouse heart membranes (lanes 4 and 8):1-4. Anti-GIPR (extracellular) Antibody (#AGR-025), (1:200).
5-8. Anti-GIPR (extracellular) Antibody, preincubated with GIPR (extracellular) Blocking Peptide (#BLP-GR025).
Expression of Gastric inhibitory polypeptide receptor in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GIPR (extracellular) Antibody (#AGR-025), (1:400), followed by anti-rabbit-Alexa-488 antibody. GIPR is expressed (green) in the pyramidal layer (P) of the CA1 rat hippocampal region. Nuclei are labeled with DAPI (blue).
Properties
Sequence
- (C)EGQTTGELYQRWER,corresponding to amino acid residues 25-38 of rat GIPR (Accession P43219).
Accession (Uniprot) Number P43219
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
