Size: 0.12 mg
Type: GST fusion protein
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- GST fusion protein with the sequence ELANRAEVPLSWSVS SKLNQHAELETEEEEKNPEELTERNG, corresponding to residues 374-414 of mouse Kir3.2 (Accession P48542).
Accession (Uniprot) Number P48542
Peptide Confirmation Confirmed by DNA sequence and SDSPAGE.
Purity >95% (SDS-PAGE)
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 μl PBS.
Concentration After Reconstitution 1.2 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 µg fusion protein per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-GIRK2 (Kir3.2) Antibody (#APC-006), (1:200).
2. Anti-GIRK2 (Kir3.2) Antibody, preincubated with GIRK2 (Kir3.2) Blocking peptide (#BLP-PC006).- Rat brain and mouse brain sections (1:400).
Mouse spinal cords (Marker, C.L. et al. (2004) J. Neurosci. 24, 2806.). 
Western blot analysis of rat brain membrane (lanes 1 and 3) and mouse brain membrane (lanes 2 and 4):1,2. Guinea pig Anti-GIRK2 (Kir3.2) Antibody (#APC-006-GP), (1:400).
3,4. Guinea pig Anti-GIRK2 (Kir3.2) Antibody, preincubated with GIRK2/Kir3.2 Blocking Peptide (#BLP-PC006).
Following a screen of several secondary antibodies, the following were used for western blot analysis:
Anti-Guinea pig IgG (Sigma #A7289 or Jackson ImmunoResearch #106-035-006).- Rat and mouse brain sections (1:400).
Last update: 02/01/2024
