Overview
Type: Synthetic peptide
Form: Lyophilized powder
GLP2R (extracellular) Blocking Peptide (#BLP-GR022) is the original antigen used for immunization during Anti-GLP2R (extracellular) Antibody (#AGR-022) generation. The blocking peptide binds and ‘blocks’ Anti-GLP2R (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat colon lysate:1. Anti-GLP2R (extracellular) Antibody (#AGR-022), (1:200).
2. Anti-GLP2R (extracellular) Antibody, preincubated with GLP2R (extracellular) Blocking Peptide (#BLP-GR022).
Expression of Glucagon-like peptide 2 receptor in rat hypothalamusImmunohistochemical staining of rat hypothalamus with Anti-GLP2R (extracellular) Antibody (#AGR-022), (1:200). Glucagon-like peptide 2 receptor (green) appears in the lateral hypothalamic region axonal profiles (vertical white arrow) and nerve cell profiles (horizontal white arrows) were labeled. DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)KRPDDESGWMSYLSE, corresponding to amino acid residues 241-255 of rat GLP2R (Accession Q9Z0W0).
Accession (Uniprot) Number Q9Z0W0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
