Overview
Type: Synthetic peptide
Form: Lyophilized powder
GluR2/GluA2 (extracellular) Blocking Peptide (#BLP-GC005) is the original antigen used for immunization during Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005) generation. The blocking peptide binds and ‘blocks’ Anti-GluR2/GluA2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005), (1:200).
2. Anti-GluR2 (GluA2) (extracellular) Antibody, preincubated with GluR2/GluA2 (extracellular) Blocking Peptide (#BLP-GC005).
Expression of AMPA Receptor 2 (GluA2) in rat cerebellumImmnunohistochemical staining of rat cerebellum using Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005) . A. Co-localization of GluA2 (green) and calbindin D28-K (red) in Purkinje cell dendrites (vertical arrow). B. Distribution of calbindin D28-K. C. Distribution of GluA2. Note that some GluA2 positive fibers (horizontal arrow) that are probable Bergmann glial processes do not stain for GluA2. D. The molecular and Purkinje layer (blue).
Western blot analysis of rat brain membranes:1. Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005-GP), (1:500).
2. Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody, preincubated with GluR2/GluA2 (extracellular) Blocking Peptide (#BLP-GC005).
Western blot analysis of mouse brain lysate:1. Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005-GP), (1:600).
2. Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody, preincubated with GluR2/GluA2 (extracellular) Blocking Peptide (#BLP-GC005).
Properties
Sequence
- NVGNINNDKKDETYR(C), corresponding to amino acid residues 179-193 of rat GluR2 (Accession P19491).
Accession (Uniprot) Number P19491
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
