Overview
Type: Synthetic peptide
Form: Lyophilized powder
GluR3/GluA3 (extracellular) Blocking Peptide (#BLP-GC010) is the original antigen used for immunization during Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010) generation. The blocking peptide binds and ‘blocks’ Anti-GluR3/GluA3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat cerebellum lysates:1. Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010), (1:400).
2. Anti-GluR3 (GluA3) (extracellular) Antibody, preincubated with GluR3/GluA3 (extracellular) Blocking Peptide (#BLP-GC010).
Expression of GRIA3 (GluR3) in mouse cerebellumImmunohistochemical staining of frozen perfusion-fixed free floating sections of mouse cerebellum using Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010). A. Distribution of GRIA3 (red). B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that GRIA3 is localized to Bergmann glia (vertical arrow) and to Purkinje cell soma (horizontal arrow). DAPI is used as the counterstain (blue).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes, human SH-SY5Y neuroblastoma cell line (lanes 5 and 7) and human Jurkat T-cell leukemia cell line (lanes 6 and 8) lysates:1,2. Guinea pig Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010-GP) (1:2000).
5,6. Guinea pig Anti-GluR3 (GluA3) (extracellular) Antibody (1:400).
3,4,7,8. Guinea pig Anti-GluR3 (GluA3) (extracellular) Antibody, preincubated with GluR3/GluA3 (extracellular) Blocking Peptide (#BLP-GC010)
Expression of GluR3 (GluA3) in mouse brainImmunohistochemical staining of mouse brain sections using Guinea pig Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010-GP). A. GluA3 immunoreactivity (red) in the cerebellum appears in Purkinje cell soma (horizontal arrow) and dendrites (vertical arrow). B. Parvalbumin staining (green) appears in Purkinje cells. C. Merged image of panels A and B demonstrates partial colocalization some Purkinje soma and dendrites (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
Properties
Sequence
- (C)EKPFHLNYHVDHLD, corresponding to amino acid residues 60-73 of rat GluR3 (Accession P19492).
Accession (Uniprot) Number P19492
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
