Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)EKPFHLNYHVDHLD, corresponding to amino acid residues 60-73 of rat GluR3 (Accession P19492).
Accession (Uniprot) Number P19492
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat cerebellum lysates:1. Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010), (1:400).
2. Anti-GluR3 (GluA3) (extracellular) Antibody, preincubated with GluR3/GluA3 (extracellular) Blocking Peptide (#BLP-GC010).- Mouse brain sections (frozen), (1:50).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes, human SH-SY5Y neuroblastoma cell line (lanes 5 and 7) and human Jurkat T-cell leukemia cell line (lanes 6 and 8) lysates:1,2. Guinea pig Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010-GP) (1:2000).
5,6. Guinea pig Anti-GluR3 (GluA3) (extracellular) Antibody (1:400).
3,4,7,8. Guinea pig Anti-GluR3 (GluA3) (extracellular) Antibody, preincubated with GluR3/GluA3 (extracellular) Blocking Peptide (#BLP-GC010)- Mouse brain sections.
Last update: 02/01/2024
