Overview
Type: Synthetic peptide
Form: Lyophilized powder
GluR4/GluA4 (extracellular) Blocking Peptide (#BLP-GC019) is the original antigen used for immunization during Anti-GluR4 (GluA4) (extracellular) Antibody (#AGC-019) generation. The blocking peptide binds and ‘blocks’ Anti-GluR4/GluA4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1,2. Anti-GluR4 (GluA4) (extracellular) Antibody (#AGC-019), (1:200).
3,4. Anti-GluR4 (GluA4) (extracellular) Antibody, preincubated with GluR4/GluA4 (extracellular) Blocking Peptide (#BLP-GC019).
Expression of GRIA4 (GluR4) in rat DRGImmunohistochemical staining of rat frozen dorsal root ganglion (DRG) sections using Anti-GluR4 (GluA4) (extracellular) Antibody (#AGC-019), (1:100). GRIA4 is expressed in all the DRG neurons (red). Hoechst 33342 is used as the counterstain (blue).
Properties
Sequence
- (C)KLMDRWKKLDQRE, corresponding to amino acid residues 274-286 of rat GluR4 (Accession P19493).
Accession (Uniprot) Number P19493
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
