Overview
Type: Synthetic peptide
Form: Lyophilized powder
GLUT1 (extracellular) Blocking Peptide (#BLP-GT041) is the original antigen used for immunization during Anti-GLUT1 (extracellular) Antibody (#AGT-041) generation. The blocking peptide binds and ‘blocks’ Anti-GLUT1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-GLUT1 (extracellular) Antibody (#AGT-041), (1:400).
3,4. Anti-GLUT1 (extracellular) Antibody, preincubated with GLUT1 (extracellular) Blocking Peptide (#BLP-GT041).
Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 1 and 5), human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 6), human K562 chronic myeloid leukemia cell line lysate (lanes 3 and 7) and human Jurkat T-cell leukemia cell line lysate (lanes 4 and 8):1-4. Anti-GLUT1 (extracellular) Antibody (#AGT-041), (1:400).
5-8. Anti-GLUT1 (extracellular) Antibody, preincubated with GLUT1 (extracellular) Blocking Peptide (#BLP-GT041).
Properties
Sequence
- (C)EEFYNQTWNHRYGES, corresponding to amino acid residues 41-55 of rat Glucose transporter 1 (Accession P11167).
Accession (Uniprot) Number P11167
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
