Overview
Type: Synthetic peptide
Form: Lyophilized powder
GLUT2/SLC2A2 Blocking Peptide (#BLP-GT022) is the original antigen used for immunization during Anti-GLUT2 (SLC2A2) Antibody (#AGT-022) generation. The blocking peptide binds and ‘blocks’ Anti-GLUT2/SLC2A2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat liver membranes:1. Anti-GLUT2 (SLC2A2) Antibody (#AGT-022), (1:400).
2. Anti-GLUT2 (SLC2A2) Antibody, preincubated with GLUT2/SLC2A2 Blocking Peptide (#BLP-GT022).
Western blot analysis of mouse brain membranes:1. Anti-GLUT2 (SLC2A2) Antibody (#AGT-022), (1:200).
2. Anti-GLUT2 (SLC2A2) Antibody, preincubated with GLUT2/SLC2A2 Blocking Peptide (#BLP-GT022).
Western blot analysis of human Caco-2 colorectal adenocarcinoma cell line lysate (lanes 1 and 3) and human HepG2 liver hepatocellular carcinoma cell lysate (lanes 2 and 4):1-2. Anti-GLUT2 (SLC2A2) Antibody (#AGT-022), (1:400).
3-4. Anti-GLUT2 (SLC2A2) Antibody, preincubated with GLUT2/SLC2A2 Blocking Peptide (#BLP-GT022).
Expression of Glucose Transporter 2 in rat cerebellum and hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GLUT2 (SLC2A2) Antibody (#AGT-022), (1:120), followed by goat-anti-rabbit-AlexaFluor-488 (green). Cell nuclei are stained with DAPI (blue). A. Staining in cerebellum sections, reveals GLUT2 labeling in Purkinje cells (arrows). B. Staining in hippocampus dentate gyrus, shows labeling of both granule cells (horizontal arrow) and in hilar interneurons (vertical arrows).
Properties
Sequence
- (C)EFRKKSGSAPPRK, corresponding to amino acid residues 496-508 of rat Glucose Transporter 2 (Accession P12336).
Accession (Uniprot) Number P12336
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
