Cat #: BLP-GT022
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)EFRKKSGSAPPRK, corresponding to amino acid residues 496-508 of rat Glucose Transporter 2 (Accession P12336).
Accession (Uniprot) Number P12336
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat liver membranes:1. Anti-GLUT2 (SLC2A2) Antibody (#AGT-022), (1:400).
2. Anti-GLUT2 (SLC2A2) Antibody, preincubated with GLUT2/SLC2A2 Blocking Peptide (#BLP-GT022). - Western blot analysis of mouse brain membranes:1. Anti-GLUT2 (SLC2A2) Antibody (#AGT-022), (1:200).
2. Anti-GLUT2 (SLC2A2) Antibody, preincubated with GLUT2/SLC2A2 Blocking Peptide (#BLP-GT022). - Western blot analysis of human Caco-2 colorectal adenocarcinoma cell line lysate (lanes 1 and 3) and human HepG2 liver hepatocellular carcinoma cell lysate (lanes 2 and 4):1-2. Anti-GLUT2 (SLC2A2) Antibody (#AGT-022), (1:400).
3-4. Anti-GLUT2 (SLC2A2) Antibody, preincubated with GLUT2/SLC2A2 Blocking Peptide (#BLP-GT022). - Rat brain sections (1:120).
Last update: 02/01/2024