Overview
Type: Synthetic peptide
Form: Lyophilized powder
GLUT3 (extracellular) Blocking Peptide (#BLP-GT023) is the original antigen used for immunization during Anti-GLUT3 (extracellular) Antibody (#AGT-023) generation. The blocking peptide binds and ‘blocks’ Anti-GLUT3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1, 2. Anti-GLUT3 (extracellular) Antibody (#AGT-023), (1:400).
3, 4. Anti-GLUT3 (extracellular) Antibody, preincubated with GLUT3 (extracellular) Blocking Peptide (#BLP-GT023).
Expression of Glucose transporter 3 in mouse hippocampus and cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GLUT3 (extracellular) Antibody (#AGT-023), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GLUT3 staining (green) in mouse hippocampal dentate gyrus, is detected in interneurons (arrows pointing up) in the Hilus and granule layer (G) (arrow pointing down). B. Staining in mouse cerebellum, is seen in purkinje cells (vertical arrows) and dendrites (horizontal arrows) in the molecular layer (Mol). Cell nuclei were stained with DAPI (blue).
Properties
Sequence
- (C)KDFLNYTLEERLED, corresponding to amino acid residues 39-52 of rat Glucose Transporter 3 (Accession Q07647).
Accession (Uniprot) Number Q07647
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
